ARG11151
anti-Ubiquitin antibody [Ubi-1]
anti-Ubiquitin antibody [Ubi-1] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,C. elegans,Cow,D. melanogaster ,Horse,Monkey,Pig,Zebrafish
Overview
| Product Description | Mouse Monoclonal antibody [Ubi-1] recognizes Ubiquitin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Ce, Cow, Dm, Hrs, Mk, Pig, Zfsh |
| Predict Reactivity | Chk |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Ubi-1 |
| Isotype | IgG1 |
| Target Name | Ubiquitin |
| Antigen Species | Bovine |
| Immunogen | Purified bovine erythrocyte ubiquitin coupled to KLH with glutaraldehyde. |
| Epitope | GIPPDQQRLI |
| Conjugation | Un-conjugated |
| Alternate Names | Polyubiquitin-B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UBB |
| Gene Full Name | ubiquitin B |
| Background | This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Function | [Ubiquitin]: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. [UniProt] |
| Cellular Localization | Ubiquitin: Cytoplasm. Nucleus. [UniProt] |
| Calculated MW | 26 kDa |
| PTM | Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291). [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG11151 anti-Ubiquitin antibody [Ubi-1] WB image
Western blot: HEK293 cells untreated (left) or treated with 10 µM of proteasome inhibitor lactacystin for 16 hours (right). Cell lysates were stained with ARG11151 anti-Ubiquitin antibody [Ubi-1] (green) at 1:1000 dilution. The same blot was stained with anti-Hsp 60 antibody (red) at 1:5000 dilution, used as a loading control.
The smear detected above the 200 kDa standard represent accumulation of ubiquitinated proteins. Band at 8 kDa corresponds to monoubiquitin.
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ARG11151 anti-Ubiquitin antibody [Ubi-1] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded cerebral cortex of an Alzheimer patient stained with ARG11151 anti-Ubiquitin antibody [Ubi-1].
