ARG63336
anti-USP16 / UBP-M antibody
anti-USP16 / UBP-M antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes USP16 / UBP-M |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognise isoform a (NP_006438.1) and isoform b (NP_001001992.1). Reported variants represent identical protein (NP_006438.1; NP_001027582.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | USP16 / UBP-M |
| Antigen Species | Human |
| Immunogen | GKKRTKGKTVPIDD-C |
| Conjugation | Un-conjugated |
| Alternate Names | UBP-M; Deubiquitinating enzyme 16; Ubiquitin-processing protease UBP-M; Ubiquitin thioesterase 16; Ubiquitin-specific-processing protease 16; UBPM; EC 3.4.19.12; Ubiquitin carboxyl-terminal hydrolase 16 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Y5T5 Human Ubiquitin carboxyl-terminal hydrolase 16 |
|---|---|
| Background | This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 94 kDa |
| PTM | Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity. |
Images (1) Click the Picture to Zoom In
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ARG63336 anti-USP16 / UBP-M antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Human Kidney. (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63336 anti-USP16 / UBP-M antibody at 4 µg/ml dilution followed by HRP-staining.
