Overview

Product Description	Rabbit Polyclonal antibody recognizes USP13
Tested Reactivity	Hu, Ms
Tested Application	FACS, ICC/IF, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	USP13
Antigen Species	Human
Immunogen	Synthetic peptide of Human USP13.
Conjugation	Un-conjugated
Alternate Names	Isopeptidase T-3; Ubiquitin-specific-processing protease 13; Deubiquitinating enzyme 13; ISOT3; IsoT-3; Ubiquitin thioesterase 13; Ubiquitin carboxyl-terminal hydrolase 13; ISOT-3; EC 3.4.19.12

Application Instructions

Application Suggestion	Tested Application Dilution FACS 1:100 ICC/IF 1:50 - 1:200 IP 1:50 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	HepG2
Observed Size	~ 95 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 72607 Mouse USP13 GeneID: 8975 Human USP13 Swiss-port # Q5BKP2 Mouse Ubiquitin carboxyl-terminal hydrolase 13 Swiss-port # Q92995 Human Ubiquitin carboxyl-terminal hydrolase 13
Gene Symbol	USP13
Gene Full Name	ubiquitin specific peptidase 13 (isopeptidase T-3)
Function	Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data. [UniProt]
Calculated MW	97 kDa

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