ARG56937
anti-UNG antibody [k1C12]
anti-UNG antibody [k1C12] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [k1C12] recognizes UNG |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | k1C12 |
| Isotype | IgG2b, kappa |
| Target Name | UNG |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-313 of Human UNG. |
| Conjugation | Un-conjugated |
| Alternate Names | Uracil-DNA glycosylase; HIGM4; HIGM5; UNG1; UNG2; UNG15; UDG; DGU; EC 3.2.2.27 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UNG |
| Gene Full Name | uracil DNA glycosylase |
| Background | This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene (GeneID 10309), which has been confused with this gene, in the literature and some databases. [provided by RefSeq, Nov 2010] |
| Function | Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. [UniProt] |
| Calculated MW | 35 kDa |
| PTM | Isoform 1 is processed by cleavage of a transit peptide. |
Images (2) Click the Picture to Zoom In
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ARG56937 anti-UNG antibody [k1C12] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG56937 anti-UNG antibody [k1C12] (green) at 1:100 dilution. DAPI was stained the cell nucleus (blue).
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ARG56937 anti-UNG antibody [k1C12] WB image
Western blot: 40 µg of HeLa, Jurkat, MCF7, K562, 293T, HepG2, A549, SK-OV-3 and PC3 cell lysates stained with ARG56937 anti-UNG antibody [k1C12] at 1:1000 dilution.
