ARG45251
anti-UBE2M / UBC12 antibody
anti-UBE2M / UBC12 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Polyclonal antibody recognizes UBE2M / UBC12 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | UBE2M / UBC12 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human UBE2M / UBC12. |
| Conjugation | Un-conjugated |
| Alternate Names | UBE2M; Ubiquitin-conjugating enzyme E2 M; UBC-RS2; EC 6.3.2.-; hUbc12; UBC12; NEDD8 protein ligase; NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 21 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UBE2M |
| Gene Full Name | Ubiquitin Conjugating Enzyme E2 M |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008] |
| Function | Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation. [UniProt] |
| Cellular Localization | cytosol; glutamatergic synapse; nucleoplasm; nucleus; postsynapse; presynapse. [UniProt] |
| Calculated MW | 21 kDa |
| PTM | The acetylation of Met-1 increases affinity for DCUN1D1 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins. [UniProt] |
Images (6) Click the Picture to Zoom In
-
ARG45251 anti-UBE2M / UBC12 antibody IHC-P image
Immunohistochemistry: Human spleen stained with ARG45251 anti-UBE2M / UBC12 antibody at 2 μg/ml dilution.
-
ARG45251 anti-UBE2M / UBC12 antibody ICC/IF image
Immunofluorescence: U87 stained with ARG45251 anti-UBE2M / UBC12 antibody at 5 ug/ml dilution.
-
ARG45251 anti-UBE2M / UBC12 antibody WB image
Western blot: 293T and Ramos stained with ARG45251 anti-UBE2M / UBC12 antibody at 0.5 μg/ml dilution.
-
ARG45251 anti-UBE2M / UBC12 antibody FACS image
Flow Cytometry: Daudi stained with ARG45251 anti-UBE2M / UBC12 antibody at 1 µg/10^6 cells dilution.
-
ARG45251 anti-UBE2M / UBC12 antibody WB image
Western blot: Rat brain stained with ARG45251 anti-UBE2M / UBC12 antibody at 0.5 μg/ml dilution.
-
ARG45251 anti-UBE2M / UBC12 antibody WB image
Western blot: Mouse brain stained with ARG45251 anti-UBE2M / UBC12 antibody at 0.5 μg/ml dilution.
