ARG55988
anti-Tyrosinase antibody [T311]
anti-Tyrosinase antibody [T311] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody [T311] recognizes Tyrosinase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | T311 |
| Isotype | IgG2a, kappa |
| Target Name | Tyrosinase |
| Antigen Species | Human |
| Immunogen | Recombinant Human Tyrosinase protein. |
| Conjugation | Un-conjugated |
| Alternate Names | SHEP3; SK29-AB; Tumor rejection antigen AB; ATN; EC 1.14.18.1; Tyrosinase; LB24-AB; OCA1A; OCA1; Monophenol monooxygenase; OCAIA; CMM8; TYR |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 1 mM EDTA (pH 7.5-8.5) for 10-20 min, followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 0.1 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TYR |
| Gene Full Name | tyrosinase |
| Background | The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene. [provided by RefSeq, Oct 2008] |
| Function | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. [UniProt] |
| Cellular Localization | Cytoplasmic |
| Calculated MW | 60 kDa |
| PTM | Glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG55988 anti-Tyrosinase antibody [T311] IHC-P image
Immunohistochemistry: melanoma tissue (10X) stained with ARG55988 anti-Tyrosinase antibody [T311].
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ARG55988 anti-Tyrosinase antibody [T311] IHC-P image
Immunohistochemistry: melanoma tissue (20X) stained with ARG55988 anti-Tyrosinase antibody [T311].
