ARG10590
anti-Trypsin antibody [A16-N]
anti-Trypsin antibody [A16-N] for Western blot and Human
Overview
| Product Description | Rabbit Monoclonal antibody [A16-N] recognizes Trypsin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | A16-N |
| Isotype | IgG |
| Target Name | Trypsin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human pancreatic cationic trypsin |
| Conjugation | Un-conjugated |
| Alternate Names | Trypsin I; TRY4; TRY1; Serine protease 1; EC 3.4.21.4; Cationic trypsinogen; Trypsin-1; TRYP1; TRP1; Beta-trypsin |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 10 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PRSS1 |
| Gene Full Name | protease, serine, 1 (trypsin 1) |
| Background | This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008] |
| Function | Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. [UniProt] |
| Calculated MW | 27 kDa |
| PTM | Occurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. Sulfation at Tyr-154 increases selectivity towards basic versus apolar residues at the P2' position of inhibitors that bind in a substrate-like fashion. Although the increase in selectivity is relatively small, it may facilitate digestion of a broader range of dietary proteins. |
Images (1) Click the Picture to Zoom In
-
ARG10590 anti-Trypsin antibody [A16-N] WB image
Western blot: 20 ng, 40 ng, 100 ng, and 200 ng of Human trypsin stained with ARG10590 anti-Trypsin antibody [A16-N].
