ARG59754
anti-Tropomyosin 4 antibody
anti-Tropomyosin 4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Pig
Overview
| Product Description | Goat Polyclonal antibody recognizes Tropomyosin 4 |
|---|---|
| Tested Reactivity | Hu, Ms, Pig |
| Predict Reactivity | Cow, Rat, Dog |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Tropomyosin 4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of Human Tropomyosin 4. (NP_001138632.1; NP_003281.1. C-ENVGLHQTLDQ) |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-S-108; Tropomyosin-4; Tropomyosin alpha-4 chain; TM30p1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 32 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPM4 |
| Gene Full Name | tropomyosin 4 |
| Background | This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2009] |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Binds calcium. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Associates with F-actin stress fibers. [UniProt] |
| Calculated MW | 29 kDa |
Images (5) Click the Picture to Zoom In
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ARG59754 anti-Tropomyosin 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG59754 anti-Tropomyosin 4 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG59754 anti-Tropomyosin 4 antibody WB image
Western blot: 35 µg of Human platelet lysate (in RIPA buffer) stained with ARG59754 anti-Tropomyosin 4 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
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ARG59754 anti-Tropomyosin 4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG59754 anti-Tropomyosin 4 antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG59754 anti-Tropomyosin 4 antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG59754 anti-Tropomyosin 4 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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ARG59754 anti-Tropomyosin 4 antibody WB image
Western blot: 35 µg of Pig heart lysate (in RIPA buffer) stained with ARG59754 anti-Tropomyosin 4 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
