ARG59778
anti-Tropomyosin 2 antibody
anti-Tropomyosin 2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Tropomyosin 2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Tropomyosin 2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human Tropomyosin 2. |
| Conjugation | Un-conjugated |
| Alternate Names | Tropomyosin beta chain; Beta-tropomyosin; TMSB; AMCD1; NEM4; HEL-S-273; DA1; DA2B; Tropomyosin-2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | Mouse lung and SW620 | ||||||||||
| Observed Size | 36 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPM2 |
| Gene Full Name | tropomyosin 2 (beta) |
| Background | This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009] |
| Function | Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Note=Associates with F-actin stress fibers. [UniProt] |
| Calculated MW | 33 kDa |
| PTM | Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization (By similarity). [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG59778 anti-Tropomyosin 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis stained with ARG59778 anti-Tropomyosin 2 antibody at 1:200 dilution.
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ARG59778 anti-Tropomyosin 2 antibody WB image
Western blot: 25 µg of Mouse lung and SW620 cell lysate stained wtih ARG59778 anti-Tropomyosin 2 antibody at 1:400 dilution.
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ARG59778 anti-Tropomyosin 2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human rectal cancer stained with ARG59778 anti-Tropomyosin 2 antibody at 1:200 dilution.
