ARG66849
anti-TrkC phospho (Tyr516) antibody
anti-TrkC phospho (Tyr516) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TrkC phospho (Tyr516) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody detects endogenous levels of TrkC protein only when phosphorylated at Tyr516. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TrkC |
| Antigen Species | Human |
| Immunogen | Phosphospecific peptide around Tyr516 (aa. 482-531) of Human TrkC. |
| Conjugation | Un-conjugated |
| Alternate Names | TRKC; Neurotrophic tyrosine kinase receptor type 3; Trk-C; TrkC tyrosine kinase; NT-3 growth factor receptor; GP145-TrkC; EC 2.7.10.1; gp145(trkC) |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa + serum | ||||||
| Observed Size | ~ 150 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NTRK3 |
| Gene Full Name | neurotrophic tyrosine kinase, receptor, type 3 |
| Background | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation and may play a role in the development of proprioceptive neurons that sense body position. Mutations in this gene have been associated with medulloblastomas, secretory breast carcinomas and other cancers. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011] |
| Function | Receptor tyrosine kinase involved in nervous system and probably heart development. Upon binding of its ligand NTF3/neurotrophin-3, NTRK3 autophosphorylates and activates different signaling pathways, including the phosphatidylinositol 3-kinase/AKT and the MAPK pathways, that control cell survival and differentiation. [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. [UniProt] |
| Calculated MW | 94 kDa |
| PTM | Ligand-mediated auto-phosphorylation. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG66849 anti-TrkC phospho (Tyr516) antibody WB image
Western blot: HeLa cells treated with serum (20% for 15 min). Cell lysates were stained with ARG66849 anti-TrkC phospho (Tyr516) antibody.
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ARG66849 anti-TrkC phospho (Tyr516) antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG66849 anti-TrkC phospho (Tyr516) antibody. The picture on the right is blocked with the phospho peptide.
