ARG66294
anti-Transferrin antibody
anti-Transferrin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes Transferrin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | The antibody detects endogenous Human Transferrin protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG |
| Target Name | Transferrin |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human Transferrin. |
| Conjugation | Un-conjugated |
| Alternate Names | Beta-1 metal-binding globulin; Siderophilin; Transferrin; PRO1557; TFQTL1; Serotransferrin; PRO2086 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||
| Observed Size | ~ 77 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TF |
| Gene Full Name | transferrin |
| Background | This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009] |
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. [UniProt] |
| Calculated MW | 77 kDa |
Images (3) Click the Picture to Zoom In
-
ARG66294 anti-Transferrin antibody ICC/IF image
Immunofluorescence: Human lung cancer tissue stained with ARG66294 anti-Transferrin antibody at 1:200 dilution (4°C, overnight). Left: Target. Middle: DAPI (blue) 10min. Right: Merge.
-
ARG66294 anti-Transferrin antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver tissue stained with ARG66294 anti-Transferrin antibody at 1:200 dilution (4°C, overnight). Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0) for 20 min.
-
ARG66294 anti-Transferrin antibody WB image
Western blot: Human serum stained with ARG66294 anti-Transferrin antibody at 1:2000 dilution.
