ARG63130

anti-Tissue Factor Pathway Inhibitor antibody

anti-Tissue Factor Pathway Inhibitor antibody for Western blot and Human

Cell Biology and Cellular Response antibody

Overview

Product Description Goat Polyclonal antibody recognizes Tissue Factor Pathway Inhibitor
Tested Reactivity Hu
Tested Application WB
Specificity This antibody is expected to recognize isoform a (NP_006278.1) only.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Tissue Factor Pathway Inhibitor
Antigen Species Human
Immunogen C-VKIAYEEIFVKNM
Conjugation Un-conjugated
Alternate Names TFI; Lipoprotein-associated coagulation inhibitor; LACI; EPI; TFPI1; Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7035 Human TFPI

Swiss-port # P10646 Human Tissue factor pathway inhibitor

Background This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 35 kDa
PTM O-glycosylated.

Images (2) Click the Picture to Zoom In

  • ARG63130 anti-Tissue Factor Pathway Inhibitor antibody WB image

    Western Blot: 1). Mock transfection; 2) TFPI (RC219033) expressing plasmid transfected HEK293 cell lysate standed with ARG63130 anti-Tissue Factor Pathway Inhibitor antibody

  • ARG63130 anti-Tissue Factor Pathway Inhibitor antibody WB image

    Western Blot: HeLa lysate (RIPA buffer, 35 µg total protein cells per lane) stained with ARG63130 anti-Tissue Factor Pathway Inhibitor antibody at 0.3 µg/ml dilution.