ARG63130
anti-Tissue Factor Pathway Inhibitor antibody
anti-Tissue Factor Pathway Inhibitor antibody for Western blot and Human
Cell Biology and Cellular Response antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Tissue Factor Pathway Inhibitor |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody is expected to recognize isoform a (NP_006278.1) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Tissue Factor Pathway Inhibitor |
| Antigen Species | Human |
| Immunogen | C-VKIAYEEIFVKNM |
| Conjugation | Un-conjugated |
| Alternate Names | TFI; Lipoprotein-associated coagulation inhibitor; LACI; EPI; TFPI1; Tissue factor pathway inhibitor; TFPI; Extrinsic pathway inhibitor |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody |
| Calculated MW | 35 kDa |
| PTM | O-glycosylated. |
Images (2) Click the Picture to Zoom In
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ARG63130 anti-Tissue Factor Pathway Inhibitor antibody WB image
Western Blot: 1). Mock transfection; 2) TFPI (RC219033) expressing plasmid transfected HEK293 cell lysate standed with ARG63130 anti-Tissue Factor Pathway Inhibitor antibody
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ARG63130 anti-Tissue Factor Pathway Inhibitor antibody WB image
Western Blot: HeLa lysate (RIPA buffer, 35 µg total protein cells per lane) stained with ARG63130 anti-Tissue Factor Pathway Inhibitor antibody at 0.3 µg/ml dilution.
