ARG63112

anti-Thyroglobulin antibody [TSH-51]

anti-Thyroglobulin antibody [TSH-51] for ELISA,ICC/IF,Radioimmunoassay and Human

Cancer antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [TSH-51] recognizes Thyroglobulin

Tested Reactivity Hu
Tested Application ELISA, ICC/IF, RIA
Specificity The clone TSH-51 reacts with human thyroid stimulating hormone (hTSH, thyrotropin), a glycoprotein hormone produced by the anterior pituitary gland cells in response to signals from the hypothalamus gland in the brain.
The TSH-51 antibody reacts with association constant 5.5 x 1010 l/mol.
Following cross-reactivity expressed as binding of labelled hormone (% of total) was determined by solid phase RIA with excess of the antibody TSH-51: hTSH (68.6), hCG (0.03), hLH (2.99), hFSH (0.66).
Host Mouse
Clonality Monoclonal
Clone TSH-51
Isotype IgG2a
Target Name Thyroglobulin
Antigen Species Human
Immunogen Human thyrotropin.
Conjugation Un-conjugated
Alternate Names AITD3; Tg; TGN; Thyroglobulin

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
RIAAssay-dependent
Application Note RIA: The clone TSH-51 is suitable in combination with the clone TSH-116 (ARG63113) for immunometric assays in the screening of neonatal hypothyroidism.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7038 Human TG

Swiss-port # P01266 Human Thyroglobulin

Gene Symbol TG
Gene Full Name thyroglobulin
Background Thyrotropin (hTSH) promotes the growth of the thyroid gland in the neck and stimulates it to produce more thyroid hormones. hTSH is composed of two subunits - alpha nad beta.
Function Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). [UniProt]
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 305 kDa
PTM Sulfated tyrosines are desulfated during iodination.