ARG54581
anti-Thymidine Kinase antibody [3B3.E11]
anti-Thymidine Kinase antibody [3B3.E11] for ELISA,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [3B3.E11] recognizes Thymidine Kinase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, WB |
| Specificity | This antibody specifically recognizes human thymidine kinase. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3B3.E11 |
| Isotype | IgG1 |
| Target Name | Thymidine Kinase |
| Antigen Species | Human |
| Immunogen | 5’-His-tagged recombinanthuman thymidine kinase |
| Conjugation | Un-conjugated |
| Alternate Names | Thymidine kinase, cytosolic; TK2; EC 2.7.1.21 |
Application Instructions
| Application Note | Western blot: antibody at 10 ug/ml will recognize TK in 5, 10, or 20 ug lysates of cells that express TK. ELISA: use antibody at 2 - 5 ug/ml (optimized for TK on solid phase at 10 ug/ml). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Purification | Protein G-purified |
| Buffer | PBS (pH 7.4) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TK1 |
| Gene Full Name | thymidine kinase 1, soluble |
| Background | Thymidine kinase (TK) belongs to agroup of enzymes such as dihydrofolatereductase, thymidylate synthase, andDNA polymerase that are involved inDNA synthesis and precursorproduction. High levels of theseenzymes are present in proliferatingcells, and low levels are found in restingcells, due to multiple regulatorymechanisms that ensure exclusiveexpression of these enzymes inreplicating cells. TK is responsible forcatalyzing the phosphorylation ofthymidine, which functions as a part ofthe pyrimidine salvage pathway involvedin DNA synthesis. The activities ofenzymes such as TK are essential forthe activation of severalchemotherapeutically importantnucleoside analogues that areadministered as prodrugs. |
| Research Area | Gene Regulation antibody |
| Calculated MW | 25 kDa |
| PTM | Phosphorylated on Ser-13 in mitosis. |
Clone References
Mechanism of action of the Aurora kinase inhibitor CCT129202 and in vivo quantification of biological activity.
Fluorodeoxyuridine modulates cellular expression of the DNA base excision repair enzyme uracil-DNA glycosylase.
WB / Human
IKK alpha regulates estrogen-induced cell cycle progression by modulating E2F1 expression.
Isolation and characterization of Dictyostelium thymidine kinase 1 as a calmodulin-binding protein.
WB / Dictyostelium discoideum
