ARG45629
anti-Tamm Horsfall Glycoprotein antibody
anti-Tamm Horsfall Glycoprotein antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Tamm Horsfall Glycoprotein |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Tamm Horsfall Glycoprotein |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human Tamm Horsfall Glycoprotein. |
| Conjugation | Un-conjugated |
| Alternate Names | UMOD; Uromodulin; HNFJ; MCKD2; THGP; HNFJ1; FJHN; THP; Tamm-Horsfall urinary glycoprotein; ADMCKD2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | UMOD |
| Gene Full Name | uromodulin |
| Background | The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Uromodulin: Functions in biogenesis and organization of the apical membrane of epithelial cells of the thick ascending limb of Henle's loop (TALH), where it promotes formation of complex filamentous gel-like structure providing the water barrier permeability. May serve as a receptor for binding and endocytosis for cytokines (IL-1, IL-2) and TNF. Facilitates neutrophil migration across renal epithelial. Uromodulin, secreted form: Secreted into urine after proteolytically cleaveage. Into the urine, may contribute to colloid osmotic pressure, retards passage of positively charged electrolytes, prevents urinary tract infection and modulates formation of supersaturated salts and their crystals. [UniProt] |
| Cellular Localization | Apical cell membrane; Lipid-anchor, GPI-anchor. Basolateral cell membrane; Lipid-anchor, GPI-anchor. Cell projection, cilium membrane. Note=Only a small fraction sorts to the basolateral pole of tubular epithelial cells compared to apical localization (PubMed:22776760). Secreted into urine after cleavage (PubMed:18375198, PubMed:26811476). Colocalizes with NPHP1 and KIF3A (PubMed:20172860). Uromodulin, secreted form: Secreted. Note=Detected in urine. [UniProt] |
| Calculated MW | 69 kDa |
| PTM | N-glycosylated (PubMed:19005207, PubMed:26673890, PubMed:26811476). N-glycan heterogeneity at Asn-232: Hex7HexNAc6 (major) and dHex1Hex7HexNAc6 (minor); at Asn-322: dHex1Hex6HexNAc5 (minor), dHex1Hex7HexNAc6 (major) and dHex1Hex8HexNAc7 (minor); at Asn-396: Hex6HexNAc5 (major), dHex1Hex6HexNAc5 (minor) and Hex7HexNAc6 (minor) (PubMed:22171320). Proteolytically cleaved at a conserved C-terminal proteolytic cleavage site to generate the secreted form found in urine (PubMed:18375198, PubMed:19005207). This cleavage is catalyzed by HPN (PubMed:26673890). [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45629 anti-Tamm Horsfall Glycoprotein antibody WB image
Western blot: 293T stained with ARG45629 anti-Tamm Horsfall Glycoprotein antibody at 0.5 μg/ml dilution.
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ARG45629 anti-Tamm Horsfall Glycoprotein antibody FACS image
Flow Cytometry: U937 stained with ARG45629 anti-Tamm Horsfall Glycoprotein antibody at 1 µg/10^6 cells dilution.
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ARG45629 anti-Tamm Horsfall Glycoprotein antibody IHC-P image
Immunohistochemistry: Rat kidney stained with ARG45629 anti-Tamm Horsfall Glycoprotein antibody at 2 μg/ml dilution.
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ARG45629 anti-Tamm Horsfall Glycoprotein antibody WB image
Western blot: PC-12 stained with ARG45629 anti-Tamm Horsfall Glycoprotein antibody at 0.5 μg/ml dilution.
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ARG45629 anti-Tamm Horsfall Glycoprotein antibody IHC-P image
Immunohistochemistry: Mouse kidney stained with ARG45629 anti-Tamm Horsfall Glycoprotein antibody at 2 μg/ml dilution.
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ARG45629 anti-Tamm Horsfall Glycoprotein antibody WB image
Western blot: Mouse kidney stained with ARG45629 anti-Tamm Horsfall Glycoprotein antibody at 0.5 μg/ml dilution.
