Overview

Product Description	Rabbit Polyclonal antibody recognizes TXNIP
Tested Reactivity	Hu, Ms, Rat
Tested Application	ICC/IF, IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	TXNIP
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human TXNIP.
Conjugation	Un-conjugated
Alternate Names	Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1; Thioredoxin-interacting protein; HHCPA78; VDUP1; THIF; EST01027

Application Instructions

Application Suggestion	Tested Application Dilution ICC/IF 1:50 - 1:200 IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	BxPC-3

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 10628 Human TXNIP GeneID: 117514 Rat TXNIP GeneID: 56338 Mouse TXNIP Swiss-port # Q5M7W1 Rat Thioredoxin-interacting protein Swiss-port # Q8BG60 Mouse Thioredoxin-interacting protein Swiss-port # Q9H3M7 Human Thioredoxin-interacting protein More
Gene Symbol	TXNIP
Gene Full Name	thioredoxin interacting protein
Background	This gene encodes a thioredoxin-binding protein that is a member of the alpha arrestin protein family. Thioredoxin is a thiol-oxidoreductase that is a major regulator of cellular redox signaling which protects cells from oxidative stress. This protein inhibits the antioxidative function of thioredoxin resulting in the accumulation of reactive oxygen species and cellular stress. This protein also functions as a regulator of cellular metabolism and of endoplasmic reticulum (ER) stress. This protein may also function as a tumor suppressor. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function	May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1). [UniProt]
Cellular Localization	Cytoplasm. [UniProt]
Calculated MW	44 kDa
PTM	Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation. [UniProt]

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