ARG42756
anti-TXNDC4 antibody
anti-TXNDC4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TXNDC4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TXNDC4 |
| Antigen Species | Human |
| Immunogen | Fusion protein of Human TXNDC4. |
| Conjugation | Un-conjugated |
| Alternate Names | Thioredoxin domain-containing protein 4; ERp44; Endoplasmic reticulum resident protein 44; PDIA10; TXNDC4; ER protein 44 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | WB: 231, HeLa, HepG2, A431, K562 and Raji IHC-P: Human thyroid cancer and Human prostate cancer |
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| Observed Size | ~ 47 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4), 0.05% Sodium azide and 40% Glycerol. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 40% Glycerol |
| Concentration | 0.4 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9BS26 Human Endoplasmic reticulum resident protein 44 |
|---|---|
| Gene Symbol | ERP44 |
| Gene Full Name | endoplasmic reticulum protein 44 |
| Background | This gene encodes a member of the protein disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins. It has an inferred N-terminal signal peptide, a catalytically active thioredoxin (TRX) domain, two TRX-like domains and a C-terminal ER-retention sequence. This protein functions as a pH-regulated chaperone of the secretory pathway and likely plays a role in protein quality control at the endoplasmic reticulum - Golgi interface. [provided by RefSeq, Dec 2016] |
| Function | Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1A and ERO1B in the endoplasmic reticulum. [UniProt] |
| Cellular Localization | Endoplasmic reticulum lumen. [UniProt] |
| Calculated MW | 47 kDa |
Images (3) Click the Picture to Zoom In
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ARG42756 anti-TXNDC4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human thyroid cancer tissue stained with ARG42756 anti-TXNDC4 antibody (left) at 1:30 dilution, or the same antibody pre-incubated with fusion protein (right). (Original magnification: X200).
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ARG42756 anti-TXNDC4 antibody WB image
Western blot: 40 µg of 231, HeLa, HepG2, A431, K562 and Raji cell lysates stained with ARG42756 anti-TXNDC4 antibody at 1:400 dilution.
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ARG42756 anti-TXNDC4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate cancer tissue stained with ARG42756 anti-TXNDC4 antibody (left) at 1:30 dilution, or the same antibody pre-incubated with fusion protein (right). (Original magnification: X200).
