ARG45048
anti-TUBA1A antibody [RM444]
anti-TUBA1A antibody [RM444] for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit monoclonal [RM444] recognizes TUBA1A. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody reacts to dTyr alpha Tubulin |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | RM444 |
| Isotype | IgG |
| Target Name | TUBA1A |
| Immunogen | A peptide corresponding to human dTyr a-tubulin |
| Conjugation | Un-conjugated |
| Alternate Names | TUBA1A; Tubulin Alpha 1a; TUBA3; B-ALPHA-1; Tubulin, Alpha, Brain-Specific; Tubulin Alpha-1A Chain; ubulin Alpha-3 Chain; Tubulin B-Alpha-1; FLJ25113; Alpha-Tubulin 3; Hum-A-Tub1; Hum-A-Tub2; EC 3.6.5.-; LIS3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide |
| Preservative | 0.09% sodium azide |
| Stabilizer | 50% Glycerol, 1% BSA and 0.09% |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TUBA1A |
| Gene Full Name | Tubulin Alpha 1a |
| Background | Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2017] |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. [Uniprot] |
| Cellular Localization | Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Microtubule. [Uniprot] |
| PTM | Acetylation, Isopeptide bond, Methylation, Nitration, Phosphoprotein. [Uniprot] |
