ARG43707

anti-TSP1 / Thrombospondin 1 antibody

anti-TSP1 / Thrombospondin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TSP1 / Thrombospondin 1
Tested Reactivity Hu, Ms
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TSP1 / Thrombospondin 1
Antigen Species Human
Immunogen Recombinant protein of Human TSP1 / Thrombospondin 1.
Conjugation Un-conjugated
Protein Full Name Thrombospondin 1
Alternate Names TSP1; THBS; THBS-1; Thrombospondin-1; TSP-1; TSP

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:200 - 1:500
IP1:20 - 1:50
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 160-180 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Concentration Batch dependent
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7057 Human THBS1

Swiss-port # P07996 Human Thrombospondin-1

Gene Symbol THBS1
Gene Full Name thrombospondin 1
Background The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. [provided by RefSeq, Jul 2008]
Function Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 129 kDa

Images (2) Click the Picture to Zoom In

  • ARG43707 anti-TSP1 / Thrombospondin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human Cholangiocarcinoma stained with ARG43707 anti-TSP1 / Thrombospondin 1 antibody.

  • ARG43707 anti-TSP1 / Thrombospondin 1 antibody WB image

    Western blot: MCF-7 cell lysates stained with ARG43707 anti-TSP1 / Thrombospondin 1 antibody at 1:1000 dilution.