ARG66120
anti-TSLP antibody
anti-TSLP antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TSLP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TSLP |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human TSLP. (MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ) |
| Conjugation | Un-conjugated |
| Alternate Names | Thymic stromal lymphopoietin |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TSLP |
| Gene Full Name | thymic stromal lymphopoietin |
| Background | This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012] |
| Function | Isoform 1: Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells. Isoform 2: May act as an antimicrobial peptide in the oral cavity and on the skin. [UniProt] |
| Calculated MW | 18 kDa |
Images (4) Click the Picture to Zoom In
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ARG66120 anti-TSLP antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human prostate. The recommended ARG66120 anti-TSLP antibody concentration is 0.125 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG66120 anti-TSLP antibody WB image
Western blot: 250 - 0.24 ng of Human TSLP stained with ARG66120 anti-TSLP antibody, under non-reducing conditions.
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ARG66120 anti-TSLP antibody standard curve image
Sandwich ELISA: ARG66120 anti-TSLP antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66121 anti-TSLP antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66120 anti-TSLP antibody WB image
Western blot: 250 - 0.24 ng of Human TSLP stained with ARG66120 anti-TSLP antibody, under reducing conditions.
