ARG23866
anti-TROP2 antibody [M005]
anti-TROP2 antibody [M005] for ELISA,ICC/IF,Immunoprecipitation,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [M005] recognizes TROP2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, IP, WB |
| Specificity | This antibody detects a 50-65 kDa band corresponding to the molecular mass of TROP2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured TROP2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | M005 |
| Isotype | IgG1 |
| Target Name | TROP2 |
| Antigen Species | Human |
| Immunogen | Extracellular region of Human TROP2 from the A431 epidermoid carcinoma cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | Membrane component chromosome 1 surface marker 1; M1S1; Cell surface glycoprotein Trop-2; TROP2; EGP-1; EGP1; Tumor-associated calcium signal transducer 2; GA733-1; Pancreatic carcinoma marker protein GA733-1; GA7331; GP50 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 50 - 65 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 50% Glycerol and 1 mg/ml BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P09758 Human Tumor-associated calcium signal transducer 2 |
|---|---|
| Gene Symbol | TACSTD2 |
| Gene Full Name | tumor-associated calcium signal transducer 2 |
| Background | This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009] |
| Function | May function as a growth factor receptor. [UniProt] |
| Calculated MW | 36 kDa |
| PTM | The N-terminus is blocked. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG23866 anti-TROP2 antibody [M005] ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed A431 cells stained with ARG23866 anti-TROP2 antibody [M005] (red).
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ARG23866 anti-TROP2 antibody [M005] WB image
Western blot: A431, MDA-MB-231 and MCF-7 cell lysates under native ("n") and denatured ("d") conditions. The blots were stained with ARG23866 anti-TROP2 antibody [M005] at 1:1000 dilution.
