ARG63888
anti-TRIM5 alpha antibody
anti-TRIM5 alpha antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TRIM5 alpha |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognise isoform alpha (NP_149023.1) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRIM5 alpha |
| Antigen Species | Human |
| Immunogen | CNIEKNENYQPK |
| Conjugation | Un-conjugated |
| Alternate Names | TRIM5; Tripartite Motif Containing 5; RNF88; TRIM5alpha; RING-Type E3 Ubiquitin Transferase TRIM5; Tripartite Motif-Containing Protein 5; Tripartite Motif Protein TRIM5; Tripartite Motif-Containing Protein 5 Alpha; Tripartite Motif Protein TRIM; Tripartite Motif-Containing 5; Ring Finger Protein 88; RING Finger Protein 88 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9C035 Human Tripartite motif-containing protein 5 |
|---|---|
| Gene Symbol | TRIM5 |
| Gene Full Name | Tripartite Motif Containing 5 |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Function | Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction factor, also acts as a pattern recognition receptor that activates innate immune signaling in response to the retroviral capsid lattice. Binding to the viral capsid triggers its E3 ubiquitin ligase activity, and in concert with the heterodimeric ubiquitin conjugating enzyme complex UBE2V1-UBE2N (also known as UBC13-UEV1A complex) generates 'Lys-63'-linked polyubiquitin chains, which in turn are catalysts in the autophosphorylation of the MAP3K7/TAK1 complex (includes TAK1, TAB2, and TAB3). Activation of the MAP3K7/TAK1 complex by autophosphorylation results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes, thereby leading to an innate immune response in the infected cell. Restricts infection by N-tropic murine leukemia virus (N-MLV), equine infectious anemia virus (EIAV), simian immunodeficiency virus of macaques (SIVmac), feline immunodeficiency virus (FIV), and bovine immunodeficiency virus (BIV). |
| Cellular Localization | Cytoplasm, Nucleus |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 56 kDa |
| PTM | Acetylation, Phosphoprotein, Ubl conjugation |
Images (1) Click the Picture to Zoom In
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ARG63888 anti-TRIM5 alpha antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63888 anti-TRIM5 alpha antibody at 4 µg/ml dilution followed by HRP-staining.
