ARG63310
anti-TRIM5 / RNF88 antibody
anti-TRIM5 / RNF88 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TRIM5 / RNF88 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise all three human isoforms of this protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRIM5 / RNF88 |
| Antigen Species | Human |
| Immunogen | ASGILVNVKEEVTC |
| Conjugation | Un-conjugated |
| Alternate Names | RING finger protein 88; RNF88; TRIM5alpha; EC 6.3.2.-; Tripartite motif-containing protein 5 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 37, 56 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9C035 Human Tripartite motif-containing protein 5 |
|---|---|
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009] |
| Research Area | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody |
| Calculated MW | 56 kDa |
| PTM | Degraded in a proteasome-independent fashion in the absence of viral infection but in a proteasome-dependent fashion following exposure to restriction sensitive virus. Autoubiquitinated in a RING finger- and UBE2D2-dependent manner. Monoubiquitinated by TRIM21. Deubiquitinated by Yersinia YopJ. Ubiquitination may not lead to proteasomal degradation. |
Images (3) Click the Picture to Zoom In
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ARG63310 anti-TRIM5 / RNF88 antibody WB image
Western Blot: Human Placenta lysate (35 µg protein in RIPA buffer) stained with ARG63310 anti-TRIM5 / RNF88 antibody at 2 µg/ml dilution.
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ARG63310 anti-TRIM5 / RNF88 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63310 anti-TRIM5 / RNF88 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63310 anti-TRIM5 / RNF88 antibody WB image
Western Blot: Human Thymus lysate (35 µg protein in RIPA buffer) stained with ARG63310 anti-TRIM5 / RNF88 antibody at 1 µg/ml dilution.
