ARG45403
anti-TRIM33 / TIF1 gamma antibody
anti-TRIM33 / TIF1 gamma antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TRIM33 / TIF1 gamma |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRIM33 / TIF1 gamma |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human TRIM33 / TIF1 gamma. |
| Conjugation | Un-conjugated |
| Alternate Names | TIF1GAMMA; Ectodermin homolog; EC 6.3.2.-; Transcription intermediary factor 1-gamma; TIFGAMMA; RET-fused gene 7 protein; E3 ubiquitin-protein ligase TRIM33; TIF1G; TIF1-gamma; Protein Rfg7; PTC7; RFG7; Tripartite motif-containing protein 33; ECTO; TF1G |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 150 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q99PP7 Mouse E3 ubiquitin-protein ligase TRIM33 Swiss-port # Q9UPN9 Human E3 ubiquitin-protein ligase TRIM33 |
|---|---|
| Gene Symbol | TRIM33 |
| Gene Full Name | tripartite motif containing 33 |
| Background | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). |
| Function | Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 123 kDa |
| PTM | Sumoylated with SUMO1. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45403 anti-TRIM33 / TIF1 gamma antibody IHC-P image
Immunohistochemistry: Human lung adenocarcinoma stained with ARG45403 anti-TRIM33 / TIF1 gamma antibody at 2 μg/ml dilution.
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ARG45403 anti-TRIM33 / TIF1 gamma antibody ICC/IF image
Immunofluorescence: A431 stained with ARG45403 anti-TRIM33 / TIF1 gamma antibody at 2 μg/ml dilution.
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ARG45403 anti-TRIM33 / TIF1 gamma antibody WB image
Western blot: K562 stained with ARG45403 anti-TRIM33 / TIF1 gamma antibody at 0.5 μg/ml dilution.
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ARG45403 anti-TRIM33 / TIF1 gamma antibody FACS image
Flow Cytometry: U87 stained with ARG45403 anti-TRIM33 / TIF1 gamma antibody at 1 µg/10^6 cells dilution.
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ARG45403 anti-TRIM33 / TIF1 gamma antibody WB image
Western blot: Rat brain stained with ARG45403 anti-TRIM33 / TIF1 gamma antibody at 0.5 μg/ml dilution.
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ARG45403 anti-TRIM33 / TIF1 gamma antibody WB image
Western blot: Mouse brain stained with ARG45403 anti-TRIM33 / TIF1 gamma antibody at 0.5 μg/ml dilution.
