ARG41747

anti-TRIM31 antibody

anti-TRIM31 antibody for ICC/IF,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes TRIM31
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRIM31
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 186-425 of Human TRIM31 (NP_008959.3).
Conjugation Un-conjugated
Alternate Names EC 6.3.2.-; E3 ubiquitin-protein ligase TRIM31; Tripartite motif-containing protein 31; RNF; HCGI; HCG1; C6orf13

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT-29
Observed Size ~ 48 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 11074 Human TRIM31

GeneID: 224762 Mouse TRIM31

Swiss-port # Q8R0K2 Mouse E3 ubiquitin-protein ligase TRIM31

Swiss-port # Q9BZY9 Human E3 ubiquitin-protein ligase TRIM31

Gene Symbol TRIM31
Gene Full Name tripartite motif containing 31
Background The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to both the cytoplasm and the nucleus. Its function has not been identified. [provided by RefSeq, Jul 2008]
Function Regulator of Src-induced anchorage independent cell growth (By similarity). May have E3 ubiquitin-protein ligase activity. [UniProt]
Cellular Localization Cytoplasm. Mitochondrion. Note=Predominantly expressed in the cytoplasm but a fraction is associated with the mitochondria. [UniProt]
Calculated MW 48 kDa
PTM Auto-ubiquitinated (in vitro). [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG41747 anti-TRIM31 antibody WB image

    Western blot: 25 µg of HT-29 cell lysate stained with ARG41747 anti-TRIM31 antibody at 1:1000 dilution.