ARG58245
anti-TRIM23 antibody
anti-TRIM23 antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TRIM23 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRIM23 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-280 of Human TRIM23 (NP_001647.1). |
| Conjugation | Un-conjugated |
| Alternate Names | ARFD1; EC 6.3.2.-; RNF46; Tripartite motif-containing protein 23; E3 ubiquitin-protein ligase TRIM23; ARD1; ADP-ribosylation factor domain-containing protein 1; RING finger protein 46; GTP-binding protein ARD-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | PC12 | ||||||
| Observed Size | 64 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TRIM23 |
| Gene Full Name | tripartite motif containing 23 |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq, Jul 2008] |
| Function | Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit. [UniProt] |
| Cellular Localization | Endomembrane system, Golgi apparatus membrane, Lysosome membrane. [UniProt] |
| Calculated MW | 64 kDa |
Images (1) Click the Picture to Zoom In
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ARG58245 anti-TRIM23 antibody WB image
Western blot: 25 µg of PC12 cell lysate stained with ARG58245 anti-TRIM23 antibody at 1:1000 dilution.
