ARG58835
anti-TRAP1 antibody
anti-TRAP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TRAP1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TRAP1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to A571-H704 of Human TRAP1. |
| Conjugation | Un-conjugated |
| Alternate Names | HSP90L; Tumor necrosis factor type 1 receptor-associated protein; TRAP-1; TNFR-associated protein 1; Heat shock protein 75 kDa, mitochondrial; HSP 75; HSP75 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: By heat mediation. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q12931 Human Heat shock protein 75 kDa, mitochondrial Swiss-port # Q5XHZ0 Rat Heat shock protein 75 kDa, mitochondrial |
|---|---|
| Gene Symbol | TRAP1 |
| Gene Full Name | TNF receptor-associated protein 1 |
| Background | This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
| Function | Chaperone that expresses an ATPase activity. Involved in maintaining mitochondrial function and polarization, most likely through stabilization of mitochondrial complex I. Is a negative regulator of mitochondrial respiration able to modulate the balance between oxidative phosphorylation and aerobic glycolysis. The impact of TRAP1 on mitochondrial respiration is probably mediated by modulation of mitochondrial SRC and inhibition of SDHA. [UniProt] |
| Cellular Localization | Mitochondrion. Mitochondrion inner membrane. Mitochondrion matrix. [UniProt] |
| Calculated MW | 80 kDa |
Images (2) Click the Picture to Zoom In
-
ARG58835 anti-TRAP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human intestinal cancer stained with ARG58835 anti-TRAP1 antibody.
-
ARG58835 anti-TRAP1 antibody WB image
Western blot: 50 µg of Rat cardiac muscle, 50 µg of Rat kidney, 50 µg of Rat brain, 40 µg of SMMC-7721, 40 µg of PANC and 40 µg of A549 lysates stained with ARG58835 anti-TRAP1 antibody at 0.5 µg/ml dilution.
