ARG57754
anti-TPMT antibody
anti-TPMT antibody for ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TPMT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TPMT |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-245 of Human TPMT (NP_000358.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Thiopurine methyltransferase; Thiopurine S-methyltransferase; EC 2.1.1.67 |
Application Instructions
| Predict Reactivity Note | Mouse | ||||||
|---|---|---|---|---|---|---|---|
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPMT |
| Gene Full Name | thiopurine S-methyltransferase |
| Background | This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014] |
| Function | Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 28 kDa |
Images (2) Click the Picture to Zoom In
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ARG57754 anti-TPMT antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG57754 anti-TPMT antibody.
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ARG57754 anti-TPMT antibody WB image
Western blot: 25 µg of K562 cell lysate stained with ARG57754 anti-TPMT antibody at 1:1000 dilution.
