ARG57762
anti-TPMT antibody [2E7]
anti-TPMT antibody [2E7] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2E7] recognizes TPMT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2E7 |
| Isotype | IgG1, kappa |
| Target Name | TPMT |
| Antigen Species | Human |
| Immunogen | Recombinant protein around aa. 1-245 of Human TPMT. |
| Conjugation | Un-conjugated |
| Alternate Names | Thiopurine methyltransferase; Thiopurine S-methyltransferase; EC 2.1.1.67 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPMT |
| Gene Full Name | thiopurine S-methyltransferase |
| Background | This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014] |
| Function | Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. [UniProt] |
| Calculated MW | 28 kDa |
Images (3) Click the Picture to Zoom In
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ARG57762 anti-TPMT antibody [2E7] ICC/IF image
Immunofluorescence: U87MG cells stained with ARG57762 anti-TPMT antibody [2E7] (green) at 1:100 dilution and DAPI (blue).
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ARG57762 anti-TPMT antibody [2E7] WB image
Western blot: 40 µg of Hep3B cell lysate stained with ARG57762 anti-TPMT antibody [2E7] at 1:1000 dilution.
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ARG57762 anti-TPMT antibody [2E7] WB image
Western blot: 50 ng of TPMT recombinant protein and 40 µg of HepG2 cell lysates stained with ARG57762 anti-TPMT antibody [2E7] at 1:1000 dilution.
