ARG52459
anti-TPH1 phospho (Ser260) antibody
anti-TPH1 phospho (Ser260) antibody for Western blot and Human,Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TPH1 phospho (Ser260) |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms, Bov, Chk, Dog, Zfsh |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TPH1 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser260 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Tryptophan 5-hydroxylase 1; TRPH; EC 1.14.16.4; Tryptophan 5-monooxygenase 1; TPRH |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~55k tryptophan hydroxylase protein phosphorylated at Ser260. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPH1 |
| Gene Full Name | tryptophan hydroxylase 1 |
| Background | Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser260 which lies within the regulatory domain of TPH (Jiang et al., 2000). |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 51 kDa |
Images (1) Click the Picture to Zoom In
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ARG52459 anti-TPH1 phospho (Ser260) antibody WB image
Western blot: Rat brainstem lysate showing specific immunolabeling of the ~55k TPH protein phosphorylated at Ser260 stained with ARG52459 anti-TPH1 phospho (Ser260) antibody. The labeling is specifically blocked by the phosphopeptide (Phos-pep) used as antigen. The corresponding non-phosphopeptide (Nonphos-pep) did not block the immunolabeling.
