ARG52456
anti-TPH1 antibody
anti-TPH1 antibody for IHC-Frozen sections,Western blot and Human,Rat
Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Sheep Polyclonal antibody recognizes TPH1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Mamm |
| Tested Application | IHC-Fr, WB |
| Host | Sheep |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TPH1 |
| Antigen Species | Rabbit |
| Immunogen | Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE |
| Conjugation | Un-conjugated |
| Alternate Names | Tryptophan 5-hydroxylase 1; TRPH; EC 1.14.16.4; Tryptophan 5-monooxygenase 1; TPRH |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~55k tryptophan hydroxylase protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPH1 |
| Gene Full Name | tryptophan hydroxylase 1 |
| Background | Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002) . Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001). |
| Research Area | Cancer antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 51 kDa |
Images (1) Click the Picture to Zoom In
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ARG52456 anti-TPH1 antibody WB image
Western blot: Human dorsal Raphe nucleus showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein stained with ARG52456 anti-TPH1 antibody.
