ARG41156
anti-TNNI1 antibody
anti-TNNI1 antibody for Western blot and Human,Mouse
Overview
| Product Description | Goat Polyclonal antibody recognizes TNNI1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Cow, Pig |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TNNI1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the N-terminus of Human TNNI1. (PEVERKPKITASRK-C, NP_003272.3) |
| Conjugation | Un-conjugated |
| Alternate Names | Troponin I, slow-twitch isoform; SSTNI; TNN1; Troponin I, slow skeletal muscle |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 25 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TNNI1 |
| Gene Full Name | troponin I type 1 (skeletal, slow) |
| Background | Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008] |
| Function | Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Calculated MW | 22 kDa |
Images (1) Click the Picture to Zoom In
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ARG41156 anti-TNNI1 antibody WB image
Western blot: 35 µg of Human skeletal muscle lysate (in RIPA buffer) stained with ARG41156 anti-TNNI1 antibody at 0.03 µg/ml dilution and incubated at RT for 1 hour.
