ARG65483

anti-TNF alpha antibody [MAb11] (FITC)

anti-TNF alpha antibody [MAb11] (FITC) for Flow cytometry and Human,Primates,Pig

Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [MAb11] recognizes TNF-alpha
Tested Reactivity Hu, NHuPrm, Pig
Tested Application FACS
Specificity The clone MAb11 recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Host Mouse
Clonality Monoclonal
Clone MAb11
Isotype IgG1
Target Name TNF alpha
Antigen Species Human
Immunogen Recombinant human TNF-alpha
Conjugation FITC
Alternate Names Tumor necrosis factor ligand superfamily member 2; DIF; Cachectin; ICD2; ICD1; N-terminal fragment; TNF-a; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; NTF

Application Instructions

Application Suggestion
Tested Application Dilution
FACS4 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 397086 Pig TNF

GeneID: 7124 Human TNF

Swiss-port # P01375 Human Tumor necrosis factor

Swiss-port # P23563 Pig Tumor necrosis factor

Gene Symbol TNF
Gene Full Name tumor necrosis factor
Background TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Function Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Upregulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208).
The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. [UniProt]
Highlight Related products:
TNF alpha antibodies; TNF alpha ELISA Kits; TNF alpha Duos / Panels; TNF alpha recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 26 kDa
PTM The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space.
The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Images (3) Click the Picture to Zoom In

  • ARG65483 anti-TNF alpha antibody [MAb11] (FITC) FACS image

    Flow Cytometry: PHA stimulated human peripheral blood mononuclear cells stained with ARG65483 anti-TNF alpha antibody [MAb11] (FITC) (4 µl reagent / 10^6 cells in 100 µl of cell suspension).

  • ARG65483 anti-TNF alpha antibody [MAb11] (FITC) FACS image

    Flow Cytometry: PHA stimulated human peripheral blood mononuclear cells stained with ARG65483 anti-TNF alpha antibody [MAb11] (FITC) (4 µl reagent / 10^6 cells in 100 µl of cell suspension). Cells were co-stained with ARG54302 anti-CD3 antibody [UCHT1] (APC) (10 µl reagent / 10^6 cells in 100 µl of cell suspension).

  • ARG65483 anti-TNF alpha antibody [MAb11] (FITC) FACS image

    Flow Cytometry: Separation of human TNF alpha positive CD3 positive cells (red-filled) from TNF alpha negative CD3 negative cells (black-dashed). Human PHA stimulated peripheral blood mononuclear cells stained with ARG65483 anti-TNF alpha antibody [MAb11] (FITC) (4 µl reagent / 10^6 cells in 100 µl of cell suspension).