ARG59409

anti-TMED9 antibody

anti-TMED9 antibody for Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TMED9
Tested Reactivity Hu, Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TMED9
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-224 of Human TMED9 (NP_059980.2).
Conjugation Un-conjugated
Alternate Names Glycoprotein 25L2; HSGP25L2G; p25; p24alpha2; p24 family protein alpha-2; GMP25; Transmembrane emp24 domain-containing protein 9

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat liver, Mouse lung and A-431
Observed Size 27 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 361207 Rat TMED9

GeneID: 54732 Human TMED9

GeneID: 67511 Mouse TMED9

Gene Symbol TMED9
Gene Full Name transmembrane p24 trafficking protein 9
Function Appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway. In COPI vesicle-mediated retrograde transport involved in the coatomer recruitment to membranes of the early secretory pathway. Increases coatomer-dependent activity of ARFGAP2. Thought to play a crucial role in the specific retention of p24 complexes in cis-Golgi membranes; specifically contributes to the coupled localization of TMED2 and TMED10 in the cis-Golgi network. May be involved in organization of intracellular membranes, such as of the ER-Golgi intermediate compartment and the Golgi apparatus. Involved in ER localization of PTPN2 isoform PTPB. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus, cis-Golgi network membrane; Single-pass type I membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type I membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type I membrane protein. Note=Cycles between compartments of the early secretatory pathway. [UniProt]
Calculated MW 27 kDa
PTM N-linked glycosylated containing high mannose. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG59409 anti-TMED9 antibody WB image

    Western blot: 25 µg of Rat liver, Mouse lung and A-431 cell lysates stained with ARG59409 anti-TMED9 antibody at 1:3000 dilution.