ARG63640
anti-TIRAP / Mal antibody
anti-TIRAP / Mal antibody for Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TIRAP / Mal |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This protein is expected to recognise isoform b (NP_683708.1) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TIRAP / Mal |
| Antigen Species | Human |
| Immunogen | C-EGEGERDSATVSDL |
| Conjugation | Un-conjugated |
| Alternate Names | MyD88-2; BACTS1; Toll/interleukin-1 receptor domain-containing adapter protein; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; wyatt; Mal |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P58753 Human Toll/interleukin-1 receptor domain-containing adapter protein |
|---|---|
| Background | The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 24 kDa |
| PTM | Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation. |
Images (1) Click the Picture to Zoom In
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ARG63640 anti-TIRAP / Mal antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG63640 anti-TIRAP / Mal (Isoform b) antibody at 1 µg/ml dilution.
