ARG20584
anti-TIMP2 antibody [3A4]
anti-TIMP2 antibody [3A4] for IHC-Formalin-fixed paraffin-embedded sections,IHC-Whole mount,Western blot and Human,Zebrafish
Overview
| Product Description | Mouse Monoclonal antibody [3A4] recognizes TIMP2 |
|---|---|
| Tested Reactivity | Hu, Zfsh |
| Predict Reactivity | Ms, Rat, Chk, Pig, Rb |
| Tested Application | IHC-P, IHC-Wmt, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3A4 |
| Isotype | IgG2a |
| Target Name | TIMP2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from Human TIMP-2 protein and Mouse myeloma Ag8563 cells. |
| Conjugation | Un-conjugated |
| Alternate Names | TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | IHC: formalin-fixed, paraffin-embedded colonic adenocarcinoma. Western blot: rhTIMP-2, 400 ng per lane. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A/G. |
| Buffer | PBS and 0.08% Sodium azide |
| Preservative | 0.08% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TIMP2 |
| Gene Full Name | TIMP metallopeptidase inhibitor 2 |
| Background | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008] |
| Function | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt] |
| Calculated MW | 24 kDa |
| PTM | The activity of TIMP2 is dependent on the presence of disulfide bonds. |
Images (3) Click the Picture to Zoom In
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ARG20584 anti-TIMP2 antibody [3A4] WB image
Western blot: 400 ng of 1) TIMP-1, and 2) TIMP-2 recombinant protein stained with ARG20584 anti-TIMP2 antibody [3A4].
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ARG20584 anti-TIMP2 antibody [3A4] IHC-P image
Immunohistochemistry: wax-embedded Human colon stained with ARG20584 anti-TIMP2 antibody [3A4] at 1:32 dilution.
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ARG20584 anti-TIMP2 antibody [3A4] IHC-Wmt image
Immunohistochemistry: whole-mount Zebrafish embryo stained with ARG20584 anti-TIMP2 antibody [3A4].
