ARG63658
anti-TIM50 antibody
anti-TIM50 antibody for Western blot and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TIM50 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise an epitope corresponding to aa 396-409 of human TIM50. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TIM50 |
| Antigen Species | Human |
| Immunogen | CDVRTVLEHYALEDD |
| Conjugation | Un-conjugated |
| Alternate Names | TIM50L; Mitochondrial import inner membrane translocase subunit TIM50; TIM50 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q3ZCQ8 Human Mitochondrial import inner membrane translocase subunit TIM50 |
|---|---|
| Gene Symbol | TIMM50 |
| Gene Full Name | translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) |
| Function | Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Has some phosphatase activity in vitro; however such activity may not be relevant in vivo. Isoform 2 may participate in the release of snRNPs and SMN from the Cajal body. [UniProt] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 40 kDa |
Images (1) Click the Picture to Zoom In
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ARG63658 anti-TIM50 antibody WB image
Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63658 anti-TIM50 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
