ARG57744
anti-TIGIT antibody [4A11]
anti-TIGIT antibody [4A11] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [4A11] recognizes TIGIT |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | TIGIT |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the extracellular domain of Human TIGIT. |
| Conjugation | Un-conjugated |
| Alternate Names | TIGIT; T Cell Immunoreceptor With Ig And ITIM Domains; VSIG9; VSTM3; V-Set And Immunoglobulin Domain-Containing Protein 9; V-Set And Transmembrane Domain-Containing Protein 3; T-Cell Immunoreceptor With Ig And ITIM Domains; DKFZp667A205; VSIG9, VSTM3; FLJ39873; V-Set And Immunoglobulin Domain Containing 9; Washington University Cell Adhesion Molecule 3; V-Set And Transmembrane Domain Containing 3; WUCAM |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q495A1 Human T-cell immunoreceptor with Ig and ITIM domains |
|---|---|
| Gene Symbol | TIGIT |
| Gene Full Name | T cell immunoreceptor with Ig and ITIM domains |
| Background | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. |
| Function | Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. |
| Cellular Localization | Cell membrane, Membrane |
| Calculated MW | 26 kDa |
| PTM | Disulfide bond, Glycoprotein |
Images (6) Click the Picture to Zoom In
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ARG57744 anti-TIGIT antibody [4A11] ICC/IF image
Immunocytochemistry: TIGIT over expressing HEK293 cells stained with ARG57744 anti-TIGIT antibody [4A11] and control Mouse IgG antibody (corner box) at 1 µg/ml dilution.
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ARG57744 anti-TIGIT antibody [4A11] FACS image
Flow Cytometry: Untransfected (blue) and TIGIT over expressing HEK293 cells (yellow) stained with ARG57744 anti-TIGIT antibody [4A11] at 1 µg/ml dilution.
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ARG57744 anti-TIGIT antibody [4A11] IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach carcinoma tissue stained with ARG57744 anti-TIGIT antibody [4A11] and control Mouse IgG (corner box) at 2 µg/ml dilution.
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ARG57744 anti-TIGIT antibody [4A11] WB image
Western blot: TIGIT over expressing HEK293 cells stained with ARG57744 anti-TIGIT antibody [4A11] at 0.25, 0.5 or 1 µg/ml dilution.
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ARG57744 anti-TIGIT antibody [4A11] ICC/IF image
Immunofluorescence: TIGIT over expressing HEK293 cells stained with ARG57744 anti-TIGIT antibody [4A11] at 1 µg/ml dilution.
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ARG57744 anti-TIGIT antibody [4A11] IHC image
Immunohistochemistry: Human stomach carcinoma tissue stained with ARG57744 anti-TIGIT antibody [4A11] at 5 µg/ml dilution.
