ARG63588
anti-TIF1A / TRIM24 antibody
anti-TIF1A / TRIM24 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes TIF1A / TRIM24 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Cow |
| Tested Application | IHC-P |
| Specificity | This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TIF1A / TRIM24 |
| Antigen Species | Human |
| Immunogen | C-KRLKSIEERQLLK |
| Conjugation | Un-conjugated |
| Alternate Names | RNF82; EC 6.3.2.-; Transcription intermediary factor 1-alpha; TIF1A; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; Tripartite motif-containing protein 24; TIF1-alpha; hTIF1; TF1A; TIF1ALPHA; TIF1; PTC6 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O15164 Human Transcription intermediary factor 1-alpha |
|---|---|
| Background | The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 117 kDa |
| PTM | Sumoylated. |
Images (1) Click the Picture to Zoom In
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ARG63588 anti-TIF1A / TRIM24 antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Breast cancer. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63588 anti-TIF1 alpha antibody at 4 µg/ml dilution followed by HRP-staining.
