Overview

Product Description	Rabbit Polyclonal antibody recognizes TIA1
Tested Reactivity	Hu, Ms
Tested Application	IHC-P, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	TIA1
Antigen Species	Human
Immunogen	Synthetic peptide from Human TIA1.
Conjugation	Un-conjugated
Alternate Names	T-cell-restricted intracellular antigen-1; WDM; TIA-1; RNA-binding protein TIA-1; p40-TIA-1; Nucleolysin TIA-1 isoform p40

Application Instructions

Application Suggestion	Tested Application Dilution IHC-P 1:50 - 1:200 WB 1:500 - 1:2000
Application Note	IHC-P: Antigen Retrieval: Heat mediated. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size	~ 45 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 21841 Mouse TIA1 GeneID: 7072 Human TIA1 Swiss-port # P31483 Human Nucleolysin TIA-1 isoform p40 Swiss-port # P52912 Mouse Nucleolysin TIA-1
Gene Symbol	TIA1
Gene Full Name	TIA1 cytotoxic granule-associated RNA binding protein
Background	The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq, Jul 2008]
Function	Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. [UniProt]
Cellular Localization	Cytoplasmic granule, Nucleus. [UniProt]
Calculated MW	43 kDa

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