ARG41154
anti-TGM3 / TGE antibody
anti-TGM3 / TGE antibody for Western blot and Human
Overview
| Product Description | Goat Polyclonal antibody recognizes TGM3 / TGE |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TGM3 / TGE |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the internal region of Human TGM3. (C-TLEVLNEARVRKP, NP_003236.3) |
| Conjugation | Un-conjugated |
| Alternate Names | Protein-glutamine gamma-glutamyltransferase E; E; Transglutaminase-3; TGE; TGase E; TGase-3; Transglutaminase E; TG; EC 2.3.2.13 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Ammonium sulphate precipitation followed by affinity purification with immunogen. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q08188 Human Protein-glutamine gamma-glutamyltransferase E |
|---|---|
| Gene Symbol | TGM3 |
| Gene Full Name | transglutaminase 3 |
| Background | Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 77 kDa |
| PTM | Activated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG41154 anti-TGM3 / TGE antibody WB image
Western blot: 35 µg of Human tonsil lysate (in RIPA buffer) stained with ARG41154 anti-TGM3 / TGE antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
