ARG59622

anti-TFIIF beta antibody

anti-TFIIF beta antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes TFIIF beta
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TFIIF beta
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-249 of Human TFIIF beta (NP_004119.1).
Conjugation Un-conjugated
Alternate Names BTF4; TF2F2; General transcription factor IIF 30 kDa subunit; General transcription factor IIF subunit 2; EC 3.6.4.12; TFIIF-beta; RAP30; ATP-dependent helicase GTF2F2; TFIIF; Transcription initiation factor RAP30; Transcription initiation factor IIF subunit beta

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse thymus and HepG2

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2963 Human GTF2F2

GeneID: 68705 Mouse GTF2F2

Swiss-port # P13984 Human General transcription factor IIF subunit 2

Swiss-port # Q8R0A0 Mouse General transcription factor IIF subunit 2

Gene Symbol GTF2F2
Gene Full Name general transcription factor IIF, polypeptide 2, 30kDa
Function TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 28 kDa

Images (3) Click the Picture to Zoom In

  • ARG59622 anti-TFIIF beta antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG59622 anti-TFIIF beta antibody.

  • ARG59622 anti-TFIIF beta antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon stained with ARG59622 anti-TFIIF beta antibody at 1:200 dilution.

  • ARG59622 anti-TFIIF beta antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney cancer stained with ARG59622 anti-TFIIF beta antibody at 1:200 dilution.