ARG40983
anti-TFF3 / Trefoil factor 3 antibody
anti-TFF3 / Trefoil factor 3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TFF3 / Trefoil factor 3 |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TFF3 / Trefoil factor 3 |
| Antigen Species | Mouse |
| Immunogen | Recombinant protein corresponding to A23-F81 of Mouse TFF3 / Trefoil factor 3. |
| Conjugation | Un-conjugated |
| Alternate Names | P1B; TFI; ITF; Trefoil factor 3; Intestinal trefoil factor; hITF; hP1.B; Polypeptide P1.B |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFF3 |
| Gene Full Name | trefoil factor 3 (intestinal) |
| Background | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] |
| Function | Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). [UniProt] |
| Cellular Localization | Secreted, extracellular space, extracellular matrix. Cytoplasm. [UniProt] |
| Calculated MW | 10 kDa |
Images (2) Click the Picture to Zoom In
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ARG40983 anti-TFF3 / Trefoil factor 3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse small intestine tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40983 anti-TFF3 / Trefoil factor 3 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG40983 anti-TFF3 / Trefoil factor 3 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Mouse small intestine tissue lysate stained with ARG40983 anti-TFF3 / Trefoil factor 3 antibody at 0.5 µg/ml, overnight at 4°C.
