ARG40985
anti-TFF2 / Spasmolytic Polypeptide antibody
anti-TFF2 / Spasmolytic Polypeptide antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TFF2 / Spasmolytic Polypeptide |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TFF2 / Spasmolytic Polypeptide |
| Antigen Species | Rat |
| Immunogen | Recombinant protein corresponding to E24-Y129 of Rat TFF2 / Spasmolytic Polypeptide. |
| Conjugation | Un-conjugated |
| Alternate Names | Trefoil factor 2; Spasmolysin; SP; SML1; Spasmolytic polypeptide |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFF2 |
| Gene Full Name | trefoil factor 2 |
| Background | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] |
| Function | Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains (By similarity). [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Calculated MW | 14 kDa |
Images (2) Click the Picture to Zoom In
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ARG40985 anti-TFF2 / Spasmolytic Polypeptide antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat gaster tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG40985 anti-TFF2 / Spasmolytic Polypeptide antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG40985 anti-TFF2 / Spasmolytic Polypeptide antibody WB image
Western blot: 50 µg of samples under reducing conditions. Rat gaster and Mouse gaster tissue lysates stained with ARG40985 anti-TFF2 / Spasmolytic Polypeptide antibody at 0.5 µg/ml, overnight at 4°C.
