ARG40245
anti-TFEB antibody
anti-TFEB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TFEB |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TFEB |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-220 of Human TFEB (NP_009093.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Class E basic helix-loop-helix protein 35; BHLHE35; ALPHATFEB; bHLHe35; TCFEB; Transcription factor EB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Rat spleen, Mouse kidney and Raji | ||||||||
| Observed Size | ~ 67 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TFEB |
| Gene Full Name | transcription factor EB |
| Function | Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Acts as a positive regulator of autophagy by promoting expression of genes involved in autophagy. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Mainly present in the cytoplasm (PubMed:23434374). Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus (PubMed:23434374). In macrophages, translocates into the nucleus upon live S.enterica infection (PubMed:27184844). [UniProt] |
| Calculated MW | 53 kDa |
| PTM | Sumoylated; does not affect dimerization with MITF. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG40245 anti-TFEB antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG40245 anti-TFEB antibody at 1:100 dilution.
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ARG40245 anti-TFEB antibody WB image
Western blot: 25 ug of Rat spleen, Mouse kidney and Raji cell lysates stained with ARG40245 anti-TFEB antibody at 1:1000 dilution.
