ARG55400
anti-TET3 antibody
anti-TET3 antibody for Immunohistochemistry,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TET3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC, WB |
| Specificity | At least three isoforms of TET3 are known to exist. This anti-TET3 antibody is predicted to not cross react with other TET proteins. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TET3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (17 aa) within aa. 1520-1570 of Human TET3. |
| Conjugation | Un-conjugated |
| Alternate Names | Methylcytosine dioxygenase TET3; B430006D22Rik; BC037432; D230004J03Rik; EC 1.14.11.n2 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | SK-N-SH Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TET3 |
| Gene Full Name | tet methylcytosine dioxygenase 3 |
| Background | Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]). [supplied by OMIM, Nov 2010] |
| Function | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. [UniProt] |
| Cellular Localization | Chromosome, Cytoplasm, Nucleus. [UniProt] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 194 kDa |
| PTM | Isopeptide bond, Ubl conjugation. [UniProt] |
Images (2) Click the Picture to Zoom In
-
ARG55400 anti-TET3 antibody WB image
Western blot: SK-N-SH cell lysate stained with ARG55400 anti-TET3 antibody at 1 µg/ml dilution in (A) the absence and (B) the presence of blocking peptide.
-
ARG55400 anti-TET3 antibody IHC image
Immunohistochemistry: Human brain tissue stained with ARG55400 anti-TET3 antibody at 20 µg/ml dilution.
