ARG66049
anti-TECK antibody
anti-TECK antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TECK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, IHC-P, Neut, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TECK |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human TECK. (QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL) |
| Conjugation | Un-conjugated |
| Alternate Names | Chemokine TECK; Ckb15; SCYA25; Thymus-expressed chemokine; Small-inducible cytokine A25; TECK; C-C motif chemokine 25 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CCL25 |
| Gene Full Name | chemokine (C-C motif) ligand 25 |
| Background | This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
| Function | Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. [UniProt] |
| Calculated MW | 17 kDa |
Images (4) Click the Picture to Zoom In
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ARG66049 anti-TECK antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human normal small bowel. The recommended ARG66049 anti-TECK antibody concentration is 0.5 µg/ml - 1.0 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Incubate tissue section in a buffer (proteinase K) at RT for 10 min.
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ARG66049 anti-TECK antibody WB image
Western blot: 250 - 0.24 ng of Human TECK stained with ARG66049 anti-TECK antibody, under non-reducing conditions.
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ARG66049 anti-TECK antibody standard curve image
Sandwich ELISA: ARG66049 anti-TECK antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG66050 anti-TECK antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66049 anti-TECK antibody WB image
Western blot: 250 - 0.24 ng of Human TECK stained with ARG66049 anti-TECK antibody, under reducing conditions.
