ARG64788
anti-TDP43 antibody
anti-TDP43 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
Publication1
Overview
| Product Description | Goat Polyclonal antibody recognizes TDP43 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TDP43 |
| Antigen Species | Human |
| Immunogen | C-HISNAEPKHNSNRQ |
| Conjugation | Un-conjugated |
| Alternate Names | TAR DNA-binding protein 43; TDP-43; ALS10 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody |
| Calculated MW | 45 kDa |
| PTM | Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. |
Images (3) Click the Picture to Zoom In
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ARG64788 anti-TDP43 antibody WB image
Western blot: Jurkat lysate (35 µg protein in RIPA buffer) stained with ARG64788 anti-TDP43 antibody at 0.3 µg/ml dilution.
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ARG64788 anti-TDP43 antibody WB image
Western blot: Human Cerebellum (A), Frontal Cotex (B) and Hippocampus (C) lysates (35 µg protein in RIPA buffer) stained with ARG64788 anti-TDP43 antibody at 1 µg/ml dilution.
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ARG64788 anti-TDP43 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64788 anti-TDP43 antibody at 5 µg/ml dilution followed by AP-staining.
Specific References
DNA damage response defects induced by the formation of TDP-43 and mutant FUS cytoplasmic inclusions and their pharmacological rescue
ICC/IF / Human
