ARG42242

anti-TCR gamma + TCR delta antibody [V65] (PE)

anti-TCR gamma + TCR delta antibody [V65] (PE) for Flow cytometry and Rat

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [V65] recognizes TCR gamma + TCR delta
Tested Reactivity Rat
Tested Application FACS
Specificity The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Host Mouse
Clonality Monoclonal
Clone V65
Isotype IgG1, kappa
Target Name TCR gamma + TCR delta
Antigen Species Rat
Immunogen TCR alpha/beta-negative CD3-positive Rat T cell hybridoma III.89.1.4 line.
Conjugation PE
Alternate Names TCR gamma: TCRG
TCR delta: TCRD; TCRDV1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TRG; TRD
Gene Full Name T cell receptor gamma locus; T cell receptor delta locus
Background TCR gamma: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangement of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia. [provided by RefSeq, Jul 2008]
Calculated MW 19 kDa

Images (1) Click the Picture to Zoom In

  • ARG42242 anti-TCR gamma + TCR delta antibody [V65] (PE) FACS image

    Flow Cytometry: Rat peripheral blood stained with ARG42242 anti-TCR gamma + TCR delta antibody [V65] (PE) and anti-CD3 antibody (Alexa Fluor® 647)