ARG65531
anti-TCR alpha + beta antibody [R73] (PE)
anti-TCR alpha + beta antibody [R73] (PE) for Flow cytometry and Rat,Primates
Developmental Biology antibody; Immune System antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [R73] recognizes TCR alpha + beta |
|---|---|
| Tested Reactivity | Rat, NHuPrm |
| Tested Application | FACS |
| Specificity | The clone R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | R73 |
| Isotype | IgG1 |
| Target Name | TCR alpha + beta |
| Immunogen | Rat T blasts and erythrocytes |
| Conjugation | PE |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography. |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression. |
| Research Area | Developmental Biology antibody; Immune System antibody |
| Calculated MW | TCR alpha: 30 kDa TCR beta: 35 kDa |
Images (1) Click the Picture to Zoom In
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ARG65531 anti-TCR alpha + beta antibody [R73] (PE) FACS image
Flow Cytometry: Separation of unstained (black) and stained (red) Rat thymocytes. Cells were stained with ARG65531 anti-TCR alpha + beta antibody [R73] (PE) at 0.11 µg/ml dilution.
